Development of multifunctional silk fibroin/pcl based electrospun nanofiber for tissue regeneration

Abstract

Bombyx mori silk cocoon derived Silk fibroin (SF) is a natural polymer which have good quality of mechanical and elasticity properties with several therapeutic efficacy such as tissue regeneration, wound healing, antibacterial etc. In this present era, SF is broadly used in novel drug delivery system (NDDS) due to its easy purification, good biocompatibility, low immunogenicity and less reaction with loaded drugs. Since last few years, electrospun nanofiber is an emerging scaffold development technique in biomedical application due to its following properties, such as high surface area, less use of chemical cross-linking agent, high processability to incorporate different therapeutic agents to modify their functional properties. Here, in this study, green synthesized reduced graphene oxide (RGO), silver nanoparticles (AgNPs) and Calendula officinalis mother tincture have incorporated in the water in oil (W/O) emulsion of biodegradable aliphatic polyester polycaprolactone (PCL) and SF to fabricate nanofiber by electrospinning technique. In this study, incorporated RGO nanosheet has prepared by one pot green synthesis method with the help of C. officinalis mother tincture extract from graphene oxide (GO) which has confirmed by the obtained FTIR, XRD and RAMAN peak and morphological image of SEM and TEM. This green synthesized RGO has also shown a promising biocompatible, antibacterial and cell proliferation properties as a therapeutic agent. ATR-FTIR, XRD, SEM, UTM, contact angle analysis, swelling index, in vitro biodegradability studies of nanofibers have shown good physiochemical and mechanical properties. Antibacterial (E. coli and B. subtilis) and cell proliferation properties (L929, mouse fibroblast cells) have compared among RGO, C. officinalis mother tincture and AgNPs doped nanofibers where 1% AgNPs doped SF- PCL based NF has shown a promising result.