Kinetic modelling and antibacterial study of drug released from clay layers and lab synthesized transdermal patch

Abstract

In the world of medicine, a class of drug called antibiotics is used to treat bacterial infections. They function by either eradicating the bacteria or slowing their growth, so preventing the spread of the infection. Clay has become a material that shows promise for drug loading. Large surface area, ability to produce nanoscale particles or layered structures, and high drug-loading capacity are all characteristics of clay. Drug molecules can be stored in the interlayer gaps of clay minerals, where they are shielded from deterioration and given greater stability. The Clay minerals like montmorillonite, kaolinite, and halloysite have these properties. Here Gatifloxacin an antibiotic drug is loaded into montmorillonite clay layers. Characterization methods like FTIR, XRD and SEM are conducted, for further confirmation and efficacy of the loaded drug, different culture of the bacteria (Staphylococcus aureus) which is a commonly found bacteria was made and the zone of inhibition of the bacteria upon application of drug was observed. The release behaviour of medications from different drug delivery devices may be predicted using release kinetics. To characterise the release kinetics and forecast the release behaviour under various situations, mathematical models such the zero-order, first-order, Higuchi, and Korsmeyer-Peppas models are frequently utilised. Burst and sustained releases were conducted to observe which model fits the best. The drug loaded clay was incorporated into patches and their release kinetics as well as the antibacterial efficacy test were performed. It was observed that the patches were successful to inhibit the bacterial growth.